Pancreatic vessel imaging in the intact adult mouse pancreas. Three-dimensional projection (left panel and middle panel) clarified mouse pancreas with nuclear stained for DAPI (blue) and vascular immunostained for α-smooth muscle actin (green). Merged image with 3D rendering nuclei (blue, left panel) and 3D rendering vessels (green, middle panel). Scale bar, 200 μm. Representative data were chosen from five independent experiments.