Figure 8

Overexpression of mud ^Sis sufficient for converting dividing cells into binucleating ones. (A–F) Cross-sectional views of main cells in early pupal proliferating stage in which mud ^PBD(A and B), mud ^L(C and D) or mud ^S(E and F) are overexpressed. Cells in telophase (A, C and E) or interphase (B, D and F) are shown. Cells are labeled with phalloidin (magenta), anti-α-Tub antibody (green in A, C and E), anti-LamDm₀ antibody (green in B, D and F) and anti-P-H3 antibody (blue). Arrowheads in (A, C and E) indicate equatorial planes. The arrowhead in (F) indicates a binucleate cell. Scale bar in (A), 5 μm, is applicable to (A, C and E). Scale bar in (B), 5 μm, is applicable to (B, D and F). (G, G’, I, I’, K and K’) Wing imaginal discs in which FLAG::mud ^PBD(G) , FLAG::mud ^L(I) or FLAG::mud ^S(K) is induced in their dorsal compartment (labeled with GFP). Cells are labeled with phalloidin (magenta), GFP (green) and anti-FLAG antibody (blue). Gray-scale images in (G’, I’ and K’) are of the blue channels in (G, I and K). Scale bar in (G), 100 μm, is applicable to (G, G’, I, I’, K and K’). (H, J and L) Magnified views of (G, I and K) around the dorso-ventral boundaries. Magenta and green channels are shown. Cells with expression of mud ^S were enlarged in volume (L), possibly as a result of cytokinesis defects. (H’, J’ and L’) Cross-sectional images reconstructed by using a stack of confocal sections at cyan lines in (H, I and L). The epithelium with expression of mud ^S has an abnormally folded or layered structure (arrowhead in L’). Scale bar in (H), 10 μm, is applicable to (H, H’, J, J’, L and L’).