Figure 4

Loss-of-function for mud erases various characteristics of binucleation. (A) Schematic diagram of the mud transcriptional unit and three representative splicing variants of mud (coding regions are in magenta). Regions corresponding to mud ^S.IR (green) and chromosomal duplication in Dp(1;3)DC281 (yellow) are also shown. (B–G) Cross-sectional views of main cells in late anaphase (B, D and F) and telophase (C, E and G) during the binucleation stage in mutants hemizygous for mud ⁴(B and C), in knockdown for mud (D and E) and in mutants hemizygous for mud ⁴ rescued by one copy of Dp(1;3)DC281 (F and G). Cells are labeled with phalloidin (magenta), anti-α-Tub antibody (green), and anti-P-H3 antibody (blue). Arrowheads in (C, E and G) indicate equatorial planes. Scale bar in (B), 5 μm, is applicable to (B–G). (B’–G’) Schematic diagrams of (B and G). (H–J) Three representative types of spindle orientation and cell shapes (bottom). Cross-sectional views of main cells in late anaphase during the binucleation stage in mutants hemizygous for mud ⁴ are shown. Cells are labeled with phalloidin (magenta) and anti-P-H3 antibody (blue). Scale bar in (H), 5 μm, is applicable to (H–J). (H’–J’) Schematic diagrams of (H–J). (K–N) Main cells expressing Pav::GFP plus ends marker in telophase in wild-type (K,L) and mud-knockdown (M,N) cells. Cell division stage in early pupa (K, en face view) and binucleation stage in mid-pupa (L–N, cross sectional views) are shown. Cells are labeled with anti-α-Tub antibody (magenta), Pav::GFP fluorescence with anti-GFP antibody (green) and anti-P-H3 antibody (blue). Arrowheads in (K and N) and curly brackets in (L and M) indicate the localization of Pav::GFP on microtubules. Scale bar in (K), 5 μm, is applicable to (K–N).