Generation of cell type-specific monoclonal antibodies for the planarian and optimization of sample processing for immunolabeling

Table 3 Effects of sample processing on immunolabeling of histological sections

Qualitative assessment of signal:noise for tissues labeled by selected mAbs: 2G4, intestine (“int.”) and epidermis (“ep.”); 3F11, intestine; 2H3, enteric muscle; 2D2, CNS neuropil; 2C11, peripharyngeal secretory cells and projections; 3H3, epidermis. “-“ indicates negligible or non-uniform labeling, poor morphology, and/or high background. “+“ indicates moderate specificity, uniform labeling, or strong signal accompanied by elevated non-specific labeling. “++” indicates robust signal with minimal noise. “n.d.”, not done.

ISSN: 1471-213X