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Table 3 Effects of sample processing on immunolabeling of histological sections

From: Generation of cell type-specific monoclonal antibodies for the planarian and optimization of sample processing for immunolabeling

mAb Fixation protocol
HCl NAc HCl NAc HCl NAc HCl NAc HCl NAc
Formaldehyde
- Bleach AR Reduction Prot K
2G4, int. ++ + + - ++ - + + - -
2G4, ep. ++ ++ - - + + - - + +
3F11 ++ + + ++ - - + + + -
2C11 + + ++ + ++ + + - + -
2D2 + - - - - - + - - -
2H3 - - - - ++ + - - + -
3H3 ++ ++ ++ ++ ++ ++ + + + +
mAb Methacarn
2G4, int. + + - n.d. ++ ++ + - + -
2G4, ep. + + - n.d. - - ++ + ++ +
3F11 - - - n.d. - - n.d. n.d. n.d. n.d.
2C11 + ++ ++ n.d. - ++ + + + ++
2D2 - - - n.d. - - n.d. n.d. n.d. n.d.
2H3 - - - n.d. + + - - - -
3H3 ++ ++ ++ n.d. ++ ++ ++ ++ + +
  1. Qualitative assessment of signal:noise for tissues labeled by selected mAbs: 2G4, intestine (“int.”) and epidermis (“ep.”); 3F11, intestine; 2H3, enteric muscle; 2D2, CNS neuropil; 2C11, peripharyngeal secretory cells and projections; 3H3, epidermis. “-“ indicates negligible or non-uniform labeling, poor morphology, and/or high background. “+“ indicates moderate specificity, uniform labeling, or strong signal accompanied by elevated non-specific labeling. “++” indicates robust signal with minimal noise. “n.d.”, not done.