|
mAb
|
Fixation protocol
|
|---|
|
HCl
|
NAc
|
HCl
|
NAc
|
HCl
|
NAc
|
HCl
|
NAc
|
HCl
|
NAc
|
|---|
|
Formaldehyde
|
|---|
|
-
|
Bleach
|
AR
|
Reduction
|
Prot K
|
|---|
|
2G4, int.
|
++
|
+
|
+
|
-
|
++
|
-
|
+
|
+
|
-
|
-
|
|
2G4, ep.
|
++
|
++
|
-
|
-
|
+
|
+
|
-
|
-
|
+
|
+
|
|
3F11
|
++
|
+
|
+
|
++
|
-
|
-
|
+
|
+
|
+
|
-
|
|
2C11
|
+
|
+
|
++
|
+
|
++
|
+
|
+
|
-
|
+
|
-
|
|
2D2
|
+
|
-
|
-
|
-
|
-
|
-
|
+
|
-
|
-
|
-
|
|
2H3
|
-
|
-
|
-
|
-
|
++
|
+
|
-
|
-
|
+
|
-
|
|
3H3
|
++
|
++
|
++
|
++
|
++
|
++
|
+
|
+
|
+
|
+
|
|
mAb
|
Methacarn
|
|
2G4, int.
|
+
|
+
|
-
|
n.d.
|
++
|
++
|
+
|
-
|
+
|
-
|
|
2G4, ep.
|
+
|
+
|
-
|
n.d.
|
-
|
-
|
++
|
+
|
++
|
+
|
|
3F11
|
-
|
-
|
-
|
n.d.
|
-
|
-
|
n.d.
|
n.d.
|
n.d.
|
n.d.
|
|
2C11
|
+
|
++
|
++
|
n.d.
|
-
|
++
|
+
|
+
|
+
|
++
|
|
2D2
|
-
|
-
|
-
|
n.d.
|
-
|
-
|
n.d.
|
n.d.
|
n.d.
|
n.d.
|
|
2H3
|
-
|
-
|
-
|
n.d.
|
+
|
+
|
-
|
-
|
-
|
-
|
|
3H3
|
++
|
++
|
++
|
n.d.
|
++
|
++
|
++
|
++
|
+
|
+
|
- Qualitative assessment of signal:noise for tissues labeled by selected mAbs: 2G4, intestine (“int.”) and epidermis (“ep.”); 3F11, intestine; 2H3, enteric muscle; 2D2, CNS neuropil; 2C11, peripharyngeal secretory cells and projections; 3H3, epidermis. “-“ indicates negligible or non-uniform labeling, poor morphology, and/or high background. “+“ indicates moderate specificity, uniform labeling, or strong signal accompanied by elevated non-specific labeling. “++” indicates robust signal with minimal noise. “n.d.”, not done.
