Effects of fixation and other parameters on immunofluorescent labeling of non-intestinal tissues. (A) mAb 2D2 labels a subset of CNS neurons and processes after formaldehyde fixation. (B) mAb 2C11 labels cell bodies around the pharynx as well as fine processes within the pharynx (arrow). (C) mAb 3G7 labeling of the epidermal basement membrane (arrows) is negatively affected by magnesium treatment, methacarn fixation, and NAc treatment. (D) mAb 3H3 labeling of epidermal cell:cell junctions. Images taken from the posterior of the animal. Region shown is the lateral margin of the animal adjacent to the pharynx. (E) mAb 2H3 labeling of body wall and enteric musculature (arrows). Sample treatment parameters are indicated at left. In all panels, anterior is to the left. Yellow asterisks indicate conditions that yielded the most specific signal with minimal background labeling. All samples were bleached in methanol (16-20 hr). Scale bars: 100 μm (A, E); 500 μm (B); 50 μm (C, D).