Figure 1From: Generation of cell type-specific monoclonal antibodies for the planarian and optimization of sample processing for immunolabelingGeneration of monoclonal antibodies that recognize the planarian intestine and other tissues. (A) Screen strategy. (B) Preimmunization serum (1:200) did not detectably label planarian tissue. (C) Test sera (1:200) ubiquitously labeled planarian tissues, with slightly elevated intestinal labeling. (D) Pie chart depicting the number of clones (n = 384 total) in the primary screen with indicated labeling specificities. (E-X) Examples and schematics of immunolabeling from the primary screen. (E-I) mAbs that label the intestine (schematic, I). (J-N) mAbs that label the nervous system (schematic, N). (O-X) Examples of mAbs that label other non-intestinal tissues (schematics, S and X). Anterior is to the top in all images. Cell and tissue types are indicated in each panel. “CNS”, central nervous system. “VNC”, ventral nerve cord. Scale bars: 100 μm (B, C, E-H, J-M, O, T, U, and W); 50 μm (P-R); 25 μm (V).Back to article page