Figure 3From: A vertebrate-conserved cis-regulatory module for targeted expression in the main hypothalamic regulatory region for the stress responseotp ECR6 displays enhancer activity in the forebrain with expression in cells localizing within the caudal telencephalon and dorsal half of the preoptic Otpa domain. A, Confocal z-stack maximum projection of an immunohistochemically stained 5 dpf Tg(otpECR6-E1b:mmGFP) larva double-labeled for GFP (A’) and Otpa (A”). B, Reconstructed localization and variability of GFP-expressing cells. Colors represent three representative maps derived from three different animals after registration using the outlines of the Otpa signal as references (transparent surface). Abbreviations: Tel, telencephalon; PO, preoptic area; PT, posterior tuberculum; H, hypothalamus; Hb, hindbrain; Pit, pituitary; ac, anterior commissure; TeO, optic tectum; Ha, habenula; PTh, prethalamus; oc, optic chiasm; poc, postoptic commissure; r, rostral; l, lateral; d, dorsal. Scale bars: 100 μm.Back to article page