Figure 6
From: CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation
Phosphorylated GM130 is stored at ERES during maturation. Oocytes were stained for GM130 (green), P-GM130 (red), and DNA (blue) at different maturation stages. (A-C) 0 h GV stage oocyte containing a perinuclear Golgi apparatus (arrowhead). (D-F) 24 h matured MI oocyte containing a metaphase plate (arrowhead). (G-I) 44 h matured MII oocyte showing the second metaphase plate (arrowhead) and a polar body (yellow arrowhead). Arrows indicate the area that is shown enlarged in the insets. Z-projections of 3 (A-F) or 6 (G-I) consecutive sections are shown; scale bars represent 20 μm in C, F and I, and 5 μm in C' F' and I'. Arrows indicate the region of the oocyte that is shown enlarged in the insets (A'-I'). (J) Weighted mean percentage ± weighted SEM of oocytes that contain a P-GM130-labeled cortical domain at the indicated maturation times. Results from 2 independent experiments are shown.