Figure 6

Influence of Ipiwi1 RNAi on adult I. pulchra at seven and 21 days of ipiwi1 dsRNA treatment. RNAi with luciferase control dsRNA did not show any effect on the level of i piwi1 expression (A), Ipiwi1 protein (D), ipvasa expression (G) or cell proliferation (J). Ipiwi1 mRNA was abrogated after seven days or 21 days of RNAi (B, C). Ipiwi1 protein was strongly reduced after seven days of ipiwi1 RNAi (E) and was completely eliminated after 21 days of ipiwi1 RNAi (F). Ipvasa expression was still prominent after seven days of ipiwi1 RNAi (H) and became significantly reduced after 21 days of ipiwi1 dsRNA treatment (I). Cell proliferation remained high up to 21 days of ipiwi1 dsRNA treatment (K, L). The specimen in figure 7L was processed for in situ hybridization before immunocytochemistry and therefore the nuclei appear larger. This protocol however did not alter cell number. In all figures, anterior is to the left. (t) testes; (de), developing eggs. Scale bars 100 μm.