Figure 7
From: Mechanism of primitive duct formation in the pancreas and submandibular glands: a role for SDF-1
SDF-1 signaling promotes epithelial cell survival in the SMG. (A) Quantification of the number of epithelial cells positive for the cleaved caspase 3 (left panel), or phosphohistone H3 (right panel) in SMG explants cultured for 24 hours. All the epithelial cells positive for the marker were counted on every sixth sections of the explant and total number was normalised to its size. Values for control explants were set as 100. t-test: *, P < 0.05. (B) Representative whole-mount immunostaining of E-cadherin in explants cultured for two days in the presence or absence of a general caspase inhibitor. Blocking caspase activity does not affect branching activity in control or AMD3100-treated explants. Scale bars, 500 μm.