Figure 2

The pancreatic buds consist of an epithelial cell mass with peripheral lumina. (A) Whole mount immunoanalyzis of dorsal and ventral pancreatic buds at e11.5 with an anti-mucin-1 antibody. The staining delineates the apical pole of cells lining the lumen of the primitive gut tube, the pancreatic central duct (*) and its extensions. It also shows blind lumina at the periphery of the pancreatic bud. (B) Gallery of pictures from a 3D confocal acquisition on a whole-mount immunofluorescence analyzis of e11.5 dorsal pancreas with anti Mucin-1 (green) antibody. Blind peripheral lumina can be observed from b to g (white arrowheads) and from f to k (red arrowheads). (C, D) Sections of e11.5 dorsal pancreatic bud stained with antibodies against E-cadherin and mucin-1 (C) and pericentrin and GM-130 (D). Nuclei are labeled with TOPRO. Epithelial cells at the periphery of the pancreatic bud (E-cadherin, dashed line) show polarized staining of mucin-1 (arrows), pericentrin and GM-130. * indicates the central duct. Scale bars, 50 μm (A); 20 μm (B); 10 μm (C, D).