Figure 6
JAK and Notch signaling can be activated in scrib/dlg mutant FCs at the anterior. Wild type (A, D and H) and scrib2 (B, E and I) or dlgm52 (C, F, G and J) mosaic egg chambers labeled by the absent of the nuclear GFP (green), stained for nuclei (DAPI, blue) and STAT92E (red in A-C and H-J), Hnt (red in D-F) or β-gal (red in G). (A-C) STAT92E protein accumulates in the nuclei predominantly at the anterior pole of the stage 8 wild type egg chamber (A, A'). This nuclear accumulation can still be observed in a number of anterior clone cells surrounding polar cells in scrib2 (B-B") or dlgm52 (C-C") mosaic chambers at stage 8. (D-F) At stage 9 Hnt is expressed in all FCs in the wild type egg chamber (D, D'). Staining of Hnt is also evident in almost all mutant cells of the scrib2 (E-E") or dlgm52 (F-F") multilayered clone. (G) In stage 6 egg chamber with dlgm52 clone at the anterior, m7-lacZ reporter is expressed in all mutant AFCs (G-G"). (H-J) In stage 10 wild type egg chamber, STAT92E nuclear accumulation is observed in the stretched cells (H, H'). STAT92E protein can accumulate in the nuclei of scrib2 (I, I") or dlgm52(J, J") clone cells at the anterior to a level comparable with that in the wild type stretched cells. Note that the mutant cells can not flatten and migrate normally (arrows in I, I', J and J').