Figure 5
Inactivation of scrib and dlg cause aberrant anterior patterning of the follicular epithelium. Wild type (A and D) and scrib2 (B, E and G) or dlgm52 (C and F) mosaic egg chambers labeled by the absent of the nuclear GFP (green), stained for nuclei (DAPI, blue) and Slbo (red in A-C) or β-gal (red in D-G). (A-C) In the scrib2 (B-B") or dlgm52 (C-C") multilayered clones at the anterior of stage 9 egg chambers, a number of FCs located around the polar cells can express Slbo and adopt the border cell fate. (D-F) In the wild type, enhancer trap reporter MA33 specifically labels the stretched cell population, which forms a squamous epithelium over the nurse cells through morphogenesis during stage 9-10 (D, D'). No MA33 expression is found in the mutant cells of scrib2 (E, E") or dlgm52 (F, F") stretched cell clone (outlined with white dots). Note that the mutant cells do not flatten and migrate towards covering the nurse cells (E, E'). (G) Compared with the stage 10b wild type egg chamber (Fig 3E, E'), clones homozygous for scrib2 neither express the centripetal cell specific marker BB127 nor migrate in between the oocyte and nurse cells (arrow in G-G").