Figure 4
con/disp1 mutants show variable postmigratory-CNCC gene expression defects. Ventral, 60 hpf (A-D), ventral, 72 hpf (E,F) or lateral, 72 hpf (G,H) views of wild type (A,C,E,G) or con/disp1 (B,D,F,H) embryos labeled with RNA probe for dlx2a (A,B), hand2 (C,D), barx1 (E,F), msxb (G,H). (A,B) dlx2a is downregulated in dorsal domain of PA1 (D1) and posterior arches in con/disp1 mutants. (C,D) hand2 is expressed in CNCC within each con/disp1 PA. (E,F) At 72 hpf, barx1 expression marks the anterior and posterior arches in wild type embryos (E), but is downregulated in the con/disp1 D1 domain of PA1 (F). (G,H) msxb is downregulated in the D1 domain, but is expressed at wild type levels in posterior arch-residing CNCC. Scale bar: 50 μM.