Figure 4

Hh stimulation triggers Smo localization to the primary cilium. (A-C) UGSM-2 cells overexpressing myc-tagged Smo were plated at subconfluence and grown in low serum media +/- 1 nM Shh peptide, +/- 10 μM cyclopamine. Cilia and microtubules are stained with anti-acetylated tubulin (green) and Smo-myc with anti-myc (red). Cyan coloring (arrows) indicates ciliary localization of Smo that is not evident in the absence of Shh ligand or in the presence of cyclopamine. (D) UGSM-2 cells overexpressing wild type Smo (WT-Smo) or ciliary localization defective Smo (CLD-Smo) were plated at confluence and grown in low serum media +/- 1 nM Shh peptide. Following 48 hrs, RNA was isolated and gene expression was determined by Real Time RT-PCR. Data represents the mean ± standard error of three replicate experiments. * indicates p < 0.05.