Figure 4
From: Arginine methyltransferase CARM1/PRMT4 regulates endochondral ossification
CARM1 regulates chondrocytes proliferation. (A) Profiles of primary cultured chondrocytes from Tg mice were evaluated at days 0 and 6. Expression change of Cyclin D1 mRNA during growth of chondrocytes was detected by using RT-PCR. (B) Mouse chondrocyte primary cell cultures were used for for FACS analysis. Cell cycle distribution was determined by propidium iodine staining of nuclear DNA. (C) Model showing functional and physical interactions between Sox9 and beta-catenin regulated by Sox9 methylation in chondrocytes. Interaction between Sox9 and beta-catenin is inhibited by Sox9 methylation caused by CARM1. Inhibition results in chondrocyte proliferation via up-regulation of beta-catenin/Tcf-Lef activity and Cyclin D1 mRNA expression.