Figure 1

Analysis of skeletal phenotypes in CARM1-null mutant embryos. (A) Appearance of skeletons of E14.5 and E16.5 embryos stained with alcian blue (cartilage) followed by alizarin red (bone). Forelimbs from E16.5 wild type (wt) (top) and null (lower) siblings show shortened bones. (B) Histological analysis of limbs of CARM1-null mutant embryos. SafraninO staining of humerus of E16.5 embryos. (B: a & b) Boxed regions are shown at higher magnification. (C) Staining by von Kossa's method in the humerus of E16.5 heterozygous and mutant embryos. (D) BrdU incorporation of humerus of E16.5 embryos. Differences, assessed by one-way analysis of variance and an unpaired Student's t-test (*), are significant p < 0.001. (E) Whole skeletal preparations of E14.5 and E18.5 CARM1 transgenic (Tg) and wt littermates. Arrow indicates a calcificated region in the shaft of the humerus of E14.5 Tg embryos. (F) Histological analysis of limbs in E14.5 wt and Tg embryos. SafraninO staining of the humerus in E14.5 embryos. Staining using von Kossa's method visualizes mineral deposition in the humerus of E14.5 Tg embryos.