Figure 2

Expression of Sepn1 transcript and SelN protein in isolated tissues between late embryonic and post-natal stages. A: Sepn1 expression was quantified by qRT-PCR and normalized to the 18s gene. Expression in quadriceps was quantified from E18 to 15 months; in diaphragm, liver, brain and kidney from E15 to 15 months; in heart from E13 to 15 months. Only mild variations were detected, including between pre- and postnatal stages. Significant variations are indicated for two consecutive points: *, p < 0.05; **, p < 0.01. B: SelN Western blot analyses are shown for quadriceps, diaphragm, heart, liver, brain and kidney at stages E18, postnatal day 1, 2 and 6 weeks, 3 and 15 months. SelN was undetectable in liver. Actin was used as a reference. Quantifications were normalized on the expression measured at E18 and are presented below the blots. For all tissues, a striking reduction of the expression was detected between E18 and postnatal day 1. This decrease was even more marked in the following post-natal stages. Statistical analyses were performed between E18 and all other stages (**, p < 0.01). d: days, w: weeks, m: months.