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Figure 3 | BMC Developmental Biology

Figure 3

From: Nucleologenesis and embryonic genome activation are defective in interspecies cloned embryos between bovine ooplasm and rhesus monkey somatic cells

Figure 3

TEM evaluation of nucleolar developmental stages in IVF, BB-SCNT, and MB-iSCNT embryos. Primary vacuole (a1, white arrow) and both primary and secondary vacuoles (a2, white arrow head) in an IVF embryo. Both primary and secondary vacuoles (b1) and reticular nucleolus (b2, black arrow) in a BB-SCNT embryo. NPBs (c1, black arrow head) and abnormally structured nucleolus (c2, gray arrows) in an MB-iSCNT embryo. (B) Proportion of each nucleolar developmental stage in IVF, BB-SCNT, and MB-iSCNT embryos. (C) Proportion of each nucleolar development stage in both 8-cell and 16-cell stage embryos. Bar, 2 μm (A1, A2, B1, and C1); 1 μm (B2 and C2).

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