Figure 6

Whole-mount in situ hybridization of candidate genes differentially-expressed between A- and P-half-sclerotome. 56 candidate genes were tested for differential expression in A- and P-sclerotome-halves by whole-mount in situ hybridization. The resultant expression patterns were placed into four different groups based upon their certainty of differential expression. Groups 1–3 represent genes with decreasing certainty of differential expression (group 1, high certainty; group 3, low certainty), while group 4 genes show no apparent staining or no observed differential expression. Three examples from each group are shown: Group 1: bmp5, gpc6, plxna2 (green text); Group 2: crabp1, robo1, sox9 (yellow text); Group 3: mospd2, timp3, rab1 (blue text); Group 4: arhgap5, nedd4, slit2 (brown text). Table 1 and Additional File 8 present additional data for the remaining 44 genes.