Skip to main content
Figure 1 | BMC Developmental Biology

Figure 1

From: Adenomatous polyposis coli-mediated control of β-catenin is essential for both chondrogenic and osteogenic differentiation of skeletal precursors

Figure 1

Col2a1- Cre ;Rosaflox mice express Cre at sites of endochondral bone formation. (A-D) LacZ expression in Col2a1-Cre;Rosaflox embryos following Cre recombination, detected by whole-mount X-Gal staining. (A) Macroscopic picture of E12.5 Col2a1-Cre;Rosaflox embryo. (B) Transversal section of E9.5 embryo showing β-galactosidase-positive sclerotomal cells adjacent to the neural tube. (C) Transversal section of E12.5 embryo showing LacZ expression in vertebrae primordia. (D) Sagital section of E16.5 embryo showing LacZ expression in the femur. The boxed region in D is magnified in D' showing LacZ expression in the periosteum (arrow head), osteoblasts (red arrow) and osteocytes (black arrow). Ne, neuroepithelium; Sc, sclerotome; N, notochord; Fe, femur. Scale bars: 1 mm in A; 50 μm in B, D'; 100 μm in C, D.

Back to article page

BMC Developmental Biology

ISSN: 1471-213X

Contact us