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Figure 2 | BMC Developmental Biology

Figure 2

From: Activation of an AMP-activated protein kinase is involved in post-diapause development of Artemia franciscana encysted embryos

Figure 2

Location of activated AMPK in developing cysts by whole mount immunohistochemistry. Decapsulated embryos developing for 2, 4, 6, 8, 10, and 12 h were fixed in 4% paraformaldehyde, permeated by 0.1% Triton X-100, incubated with phospho-AMPK (Thr172) antibody (a – f), and subsequently detected with the AP-conjugated secondary antibody by NBT/BCIP staining. e' and f' represent control embryos that were not incubated with the primary antibody. Embryos are shown at the same magnification.

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BMC Developmental Biology

ISSN: 1471-213X

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