Figure 5
Developmental expression of Ha-eIF5C in fat body and midgut showed by immunoblotting (A) and QRT-PCR (B). A: Immunoblotting showing the expression profiles of Ha-eIF5C protein in the midgut and fat body; B: QRT-PCR analysis of Ha-eIF5C mRNA transcription in the midgut and fat body from different developmental periods. 5th-Feeding: 5th instar larvae feeding stage; 5-HCS: 5th instar larvae with head capsule slippage; 6th-0: 6th instar 0 h larvae, white head (within 1 h after ecdysis); 6th-Feeding: 6th instar larvae feeding stage; Metamorphic molting: 6th instar metamotphic molting stage; p0: 0 h pupae; p1: first day pupae; p2: second day pupae. Error bars represent the standard deviation in three replicates. An asterisk indicates that the expression of Ha-eIF5C at 6th instar 120 h had statistically significant differences from those at 5th and 6th feeding stages (Student' s t-test, *: p < 0.05).