Figure 4

Rescue of Dg loss-of-function germline clones with expression of pUASp-Dg constructs. Orb (Red); GFP (Green), DAPI (Blue) A', B', D' GFP of the corresponding stages shown in a separate channel; A", B", D" Orb staining of the corresponding stages shown in a separate channel with dotted lines which indicates the border of the oocyte. A. Dg loss-of-function germline clones (black, white arrow; hsFLP; FRT42D Dg³²³) are arrested prior to stage 6 and have disrupted oocyte polarity (absent or mislocalized Orb). B. Expression of pUASp-FL with the nanos-Gal4 driver in Dg clones partially rescues oocyte polarity in arrested clones stages 3–6 as indicated by proper localization of Orb to the posterior of 36% of the oocytes; (hsFLP; FRT42D Dg³²³; P(w+:nanosGal4:VP-16)Ab-2/pUASp-FL) C. Expression of pUASp-2WW with the nanos-Gal4 driver in Dg clones does not rescues oocyte polarity in arrested clones stages 3–6 (arrow) [as indicated by development arrest and absent Orb marker; (hsFLP; FRT42D Dg³²³; P(w+:nanosGal4:VP-16)Ab-2/pUASp-2WW)] D. Expression of pUASp-PPSG with the nanos-Gal4 driver in Dg clones rescues oocyte polarity in arrested clones stages 3–6 [as indicated by proper localization of Orb to the posterior of the oocyte; (hsFLP; FRT42D Dg³²³; P(w+:nanosGal4:VP-16)Ab-2/pUASp-PPSG)]E. Wild type egg chamber with posterior Orb localization (+/+; P(w+:nanosGal4:VP-16)Ab-2/pUASp-FL) F. FL, PPSG are able to rescue Dg loss-of-function phenotypes, while 2WW and C1 do not (Red: rescued polarity index, Green: rescued growth index).