Neural crest cell markers pattern in NB cell line subtypes. All cell line subtypes exhibited positive protein expression for GAP43, c-kit, Phox2b and ABCG2 immunostaining. Differences in the intensity and localization of these proteins can be identified in some of the cell lines (see text). For Phox2b, small squares show non-merged nuclear cell images. Blue: DAPI, Green: FITC (ABCG2, GAP43). Red: Cy3 (Phox2b). c-kit (DAB staining).