Figure 5

tat-3 reporter expression pattern. The 5' end of the tat-3 locus, location of the deleted region in tm1275 mutants (bar) and structure of the tat-3 expression cassette (A). tat-3 reporter expression in the pharyngeal-intestinal valve and in muscle and marginal, but not gland, cells of the pharynx in the lcIs471.12 line (B). GFP fluorescence in the muscle and buccal epithelial cells of the pharynx procorpus and in the XXX cells in the lcIs471.4B line (C). tat-3 reporter signals in the rectum and a tail region (D), seam cells and the hypodermis (E), the DTC (F) and the AC (G) of lcIs471.4B animals. GFP staining of the vulva at the late L4 stage in lcIs881.3.2 larvae (H). Reporter expression in the adult lcIs471.4B vulva (I). Cell labels: AC – anchor cell; DTC – distal tip cell; e1 and 2 – buccal epithelial cells; g1 – gland cell; mc – marginal cell; pm2, 5, 6 and 7 – pharyngeal muscle cells; vul – vulval cells; utse – uterine seam cell; XXX – XXX cells. Abbreviations: hn – hypodermal nucleus; pv – pharyngeal-intestinal valve; sn – seam cell nucleus.