Figure 4

tat-2 reporter expression pattern. The 5' end of the tat-2 locus, locations of the deleted regions in tm1634 and tm1773 mutants (bars) and structure of the tat-2 expression cassette (A). Expression of the tat-2 reporter in the lcIs982.4.2 line in embryos (B), in the intestine of L2 larvae (C) and in gland cells of the pharynx, the pharyngeal-intestinal valve, the excretory gland cell and the intestine of a young adult (D). GFP fluorescence in the lcEx982.2.4.5 line in the excretory pore and excretory canal cells (E). Reporter signal outlining the vulF cells in early lcIs982.4.2 L4 larvae (the third frame in the series shows an overlay of the pseudocolored UV image onto the bright light image, both enlarged with re-sampling using Photoshop; dashes extend along the contact surface between the two tissues) (F). tat-2 reporter expression during spermatogenesis (G) and in adult spermatheca (H) of lcIs982.4.2 animals. Abbreviations: eg – excretory gland cell; epc – excretory pore cell; g – pharyngeal gland cell; ga – gonad arm; ph – pharynx; pv – pharyngeal-intestinal valve; oo – oocyte; r – rectum; sp – spermatheca.