Figure 2
From: Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells
A mixed population of neural progenitors and neurons derived from a dark EB. The dark EB derived from TE03 cell line was plated on PDL/laminin and cultured in the neural differentiation medium (NDM) for 3 days. (A-C) Double-immunostaining of a hESC-defferentiated aggregate for nestin and TuJ1. Newly generated nestin+ and TuJ1+ cells are migrated radially away from the center of aggregates, forming a rim of neural cells around the EB sphere. (D-G) Higher magnification of differentiated cells in (A) showing a large number of nestin+ cells (F) and a few TuJ1+ neurons (G). To estimate the percentage of differentiated cells expressing nestin or TuJ1, the number of labeled cells was counted from a double-immunolabeled culture and normalized with the total number of cells determined by counting DAPI nuclear counterstained cells (E) that overlap phase-dark (D) cells. Scale bars: in (A-C) = 200 μm; in (D-G) 100 μm.