Figure 5
From: Generation of diverse neuronal subtypes in cloned populations of stem-like cells
Flow-cytometric demonstration of SSEA-1 immunoreactive cells (green dots) in differentiated (stage 6) NE-4C cultures. The dot plot (A) shows the SSEA-1 positive population (R2) characterized by high phyco-erythrine (PE) fluorescence (≥ 600; y-axis) against a non-specific auto fluorescence (FITC; x-axis). The histogram (B) and the statistics (C) showed an about 5% frequency of SSEA-1 positive cells among the total cells (R1; 100%) defined by forward scatter (FSC) value ≥ 200.