Expression of Groucho/TLE proteins during pancreas development

Hoffman, Brad G; Zavaglia, Bogard; Beach, Mike; Helgason, Cheryl D

doi:10.1186/1471-213X-8-81

BMC Developmental Biology

Table 1 Counts of unambiguously identified Gro/TLE family members and eh1 or WRPW domain containing transcription factors in nine pancreas SAGE libraries

From: Expression of Groucho/TLE proteins during pancreas development

Tag	Position^Φ	Accession	Symbol	Pdx1 GFP+ E10.5 306588	Pdx1 GFP+ E11.5 317716	Ngn3 GFP- E12.5 308745	Ngn3 GFP+ E12.5 320473	Ngn3 GFP+ E13.5 320473	Ngn3 GFP+ E14.5 301222	Whole E14.5 98189	Whole E18.5 81130	Islets Adult 99968	S^□
Gro/TLE family members
TGAACAAGCCTGACAAG	2	NM_011599	Tle1
ATGCAGAGCGCCACAGA	16	NM_011599	Tle1
AGAGCTGCGTGCTGTCA	2	NM_019725	Tle2	0	0	1	0	5	2	0	0	0	0.5
GAGAGCAGCAATGTGGA	1	NM_009389	Tle3	0	0	0	0	0	0	0	0	1	0.0
-	-	NM_010347	Tle4	-	-	-	-	-	-	-	-	-	-
TACTATGAGATGTCCTA	1	NM_010347	Aes	0	0	3	5	11	7	0	0	0	0.2
GGGTGTGCAGATCCCGA	1	NM_053254	Tle6	1	3	2	1	20	9	0	2	0	4.4
Transcription factors containing an eh1 or WRPW Domain
-	-	NM_007492*	Arx	-	-	-	-	-	-	-	-	-	0.04
GGAGTTTTCAAGCACTG	1	NM_010132	Emx2	2	1	0	0	0	0	0	0	0	0.09
TTAACGACAAAAAAAAA	1	NM_008259	Foxa1	0	3	0	0	0	0	0	0	0	11.46
GTGAAATCCAGGTCTCG	1	NM_010446	Foxa2	0	4	0	12	12	8	4	4	8	4.08
TAGTTTTAACAGAAAAC	2	NM_010446	Foxa2	0	1	0	0	3	2	0	0	0	3.85
CTGCTATGCACCAAGAT	1	NM_008260	Foxa3	0	0	1	1	3	1	0	0	0	0.03
GACTTGTTTTTAAATGT	4	NM_008592	Foxc1	1	0	0	0	0	0	0	0	0	0.28
GCTTTGTACAGTAGATG	1	NM_013519	Foxc2	3	0	0	0	0	0	0	4	0	0.09
GCCACGCACCAGCCCCT	1	NM_010425	Foxd3	0	0	0	0	0	0	0	0	1	0.13
CACTGCATTTGTATATA	1	NM_008235	Hes1	0	1	2	0	1	0	0	2	0	13.61
CCGCTGCGTGAGGGTAG	1	NM_019479	Hes6	32	3	5	52	13	20	1	0	1	0.04
TTGGCTTCTCTTCCAGT	1	NM_010420	Hesx1	0	0	0	0	0	0	0	0	0	0.32
TATATAGCATTACTTCT	2	NM_008245	Hhex	0	1	1	1	4	1	0	1	0	4.68
CTAAATTATTATTTAGC	1	NM_019944	Hlxb9	0	0	0	0	0	1	0	0	2	4.03
AGCATTCAGATTGCTGA	2	NM_019944	Hlxb9	0	0	0	0	2	0	0	0	0	0.47
CTGGTGCTTCACCAAGG	1	NM_010835	Msx1	10	1	15	0	0	0	0	0	0	68.93
TACACGTTCTGACAACT	1	NM_010919	Nkx2-2	1	1	0	6	14	9	0	1	2	6.98
GAACTCAGCGGAGAAAG	1	NM_008699	Nkx2-3	1	0	13	1	0	0	6	0	0	0.05
CTGGCCGCCTTCAAGCC	1	NM_008700	Nkx2-5	0	0	0	0	0	0	0	2	0	0.09
CGGGGAGGCTGTCCCCA	3	NM_008700	Nkx2-5	0	0	1	0	0	0	0	0	0	-
-	-	NM_144955*	Nkx6-1	-	-	-	-	-	-	-	-	-	1.95
CCCCGACGCCAGCCGCC	4	NM_183248	Nkx6-2	2	7	0	1	2	0	0	0	0	0.13
GCTGCCTTTGAATAAAG	1	NM_008780	Pax1	3	0	0	0	0	0	0	0	0	1.92
TTCTTAATGAGGTCAGC	3	NM_008781	Pax3	1	0	0	0	0	0	0	0	0	0.02
CCTAAAGATTCCGCCGC	1	NM_008900	Pou3f3	3	0	0	0	0	0	0	0	0	54.12
TGCAAGCTGAAACCGCT	1	NM_008901	Pou3f4	1	1	0	38	3	7	0	0	0	0.05
CAGGAGAAACCCTGAGA	1	NM_009323	Tbx15	0	0	0	0	0	1	0	0	0	0.37
GCAGCAGCGGCCGCCGC	1	NM_009324	Tbx2	0	0	5	0	0	0	0	0	0	0.77
AAACAGGTGTTAAAAAC	1	NM_020496	Tbx20	0	0	0	0	0	0	0	1	0	5.94
ACTGTCTCCTCGGGCCT	4	NM_009327	Tcf1	0	1	0	1	4	1	0	1	0	0.68
TTAAGACACATCTCTGG	1	NM_009330	Tcf2	0	1	0	0	3	1	0	0	0	0.54
CCCACCAGGCCCTCCCC	2	NM_009332	Tcf3	9	2	3	1	1	0	0	0	0	0.63
ACCAGGCCTGGAGTTCT	1	NM_009332	Tcf3	7	1	1	1	2	0	0	0	0	0.24
GGACAGATGTGAAAAGG	5	NM_013685	Tcf4	6	1	5	1	3	0	3	1	1	0.05
GTCAGATTTTTTTTGGA	1	NM_013685	Tcf4	1	1	1	2	3	0	1	1	1	35.34
GGACAGAGAGACCCAAG	1	NM_021901	Tlx1	0	0	1	0	0	0	0	0	0	0.04

^Φ Tag position is calculated by assigning the 3' most CATG in the transcript as the position 1 tag and the next 3' most CATG the position 2 tag etc.
*Tags counts are shown as tags per hundred thousand. This indicates the total number of times a given SAGE tag appears in the library per hundred thousand tags and is used to normalize for libraries of varying size
^□ S is the specificity of the tag. Specificity is calculated as described in the materials and methods.
*No unambiguous tags exist for Nkx6-1 or Arx although their expression within the pancreas is well established.

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