Figure 9
From: Expression of Groucho/TLE proteins during pancreas development
Tle2 enables Arx mediated insulin reporter suppression. Min6 cells were transfected with a Rat Insulin Promoter (RIP) EGFP reporter and with either pCMS-HA (HA) vector, pCMS-Flag-Tle2 (Tle2), pCMS-Arx (Arx), pCMS-Flag-Tle2 and pCMS-Arx (Tle2/Arx), pCMS-Arx Δeh1 (Arx Δeh1), or pCMS-Flag-Tle2 and pCMS-Arx Δeh1 (Tle2/Arx Δeh1). The average of four independent experiments is shown in (A) and representative histograms from one experiment in (B). Statistical analysis was carried out using a one way ANOVA with a Bonferroni Multiple Comparison post test to compare means. * p < 0.01 and ** p < 0.001 (n = 4 experiments with triplicate samples in each experiment).