Figure 7
From: Expression of Groucho/TLE proteins during pancreas development
Expression of Tle1, Tle2, Tle3 and Tle4, in relation to insulin and glucagon expression, in the adult pancreas. Saggital sections of adult pancreases were analyzed for the expression of (A, B) Tle1, (C, D) Tle2, (E, F) Tle3 or (G, H) Tle4 (green) in relation to (A, C, E, G) insulin or (B, D, F, H) glucagon expression (red). Merged images are shown at 200× and images highlighting regions containing cells that are insulin or glucagon positive but Gro/TLE negative are shown both merged and with the channels separated at 400x. In (I) staining with a pan-TLE antibody in relationship to (J) PCNA is shown, (K) Hoescht staining shows the location of nuclei, and (L) shows the merged image. Blue arrows indicate cells that are Gro/TLE + but not insulin+ or glucagon +, while yellow arrows indicate cells where the Gro/TLE proteins and insulin or glucagon co-localize, and green arrows indicate cells that express insulin or glucagon only. Sections were photographed at ×200.