Figure 7

Confocal images of transverse sections through the pelvic ganglia from postnatal (P) 0 day mice processed for Hu (blue), TH (green) and either TrkA (red) (A-D), TrkC (red) (E-H), GFRa1 (red) (I-L), or GFRa2 (red) (M-P); or for Hu (blue), B-FABP (green) and GFRa3 (red) (Q-T) immunohistochemistry. A sub-population of cells immuno-positive for the pan-neuronal marker Hu are also TH+ (A-B, E-F, I-J, O-N, Q-R). These Hu+/TH+ cells are distributed throughout the ganglia, sometimes forming discrete clusters (B, F), othertimes more dispersed (J, N). Strong levels of TrkA (A-D), and TrkC (E-H) immunostaining were observed on the Hu+/TH+ sub-population of cells, although Hu+/TH-negative cells were also weakly TrkC immuno-positive (E-H). In contrast, GFRα1 immunostaining was observed only on the Hu+/TH-negative population of cells, and not on the Hu+/TH+ cells (asterisks). GFRα2 immunostaining was found to be ubiquitously expressed on all Hu+ cells throughout the pelvic ganglia (M-P). GFRα3 immunostaining was found to be on the neuropil only as it did not co-localise with either the pan-neuronal marker Hu or the glial marker B-FABP (Q-T). Scale bars = 20 μm.