Figure 3

A) Expression of RSPO1 mRNA in embryonic chicken gonads over development (Embryonic days (E) (stages), assayed by qRT-PCR. Values represent mean +/- SEM. ● = female; ○ = male. B) Effect of fadrozole aromatase inhibitor on gonadal RSPO1 expression in the chicken embryo. Embryos were treated at E3.5 with 0.1 ml PBS control or 0.1 ml (1 mg) fadrozole in PBS, and examined at E8.5 and E12.5. Quantitative RT-PCR. Mean +/- SEM. ■ = female; □ = male.