Figure 2

Expression of adipocyte and osteoblast markers is reciprocally affected by BRL in RBM but not in RC cells. A Northern blot analysis of adipocyte and osteoblast markers; B Quantitative analysis by densitomtery. Adipocyte markers, LPL, aP2, and adipsin. Osteoblast markers, OPN, ALP, BSP, and OCN. RBM and RC cells were treated with BRL at 10 and 100 nM and 0.1 and 1.0 μM (indicated by increasing bar size), respectively, as shown in Fig. 1. Total RNA was harvested at appropriate days as indicated. C, RT-PCR analysis of LPL, adipsin and L32 in RC cell and RBM treated with BRL and in brown adipose tissue as a positive control. Samples of RC and RBM (in the presence of BRL at 1 μM and 100 nM, respectively) are the same as those in A. Adipo, brown adipose tissue from 21-day fetal rats. D, Real-time RT-PCR of Runx2 in RC cells treated with (+) and without (-) 1 μM BRL. Samples are the same as those in A. L32, internal control. Data are mean ± SD of triplicate samples; results are representative of a minimum of three independent experiments.