Figure 1

BRL exerts different effects on bone nodule and adipocyte colony formation in RBM versus RC cell cultures. Cells were fixed at day 17 (RBM) or day 18 (RC). A, Oil red O/von Kossa staining. B, The number of adipocyte colonies and bone nodules. BRL at 10 and 100 nM and 0.1 and 1.0 μM were used for RBM and RC cells, respectively. Data are mean ± SD of triplicate samples; results are representative of a minimum of three independent experiments. *p< 0.05 vs. vehicle.