Figure 5
From: Yolk protein is expressed in the insect testis and interacts with sperm
Expression of YP2 protein in males of S. littoralis. Western blot of different segments of male reproductive system and non-reproductive organs probed with YP2 antibody. Single protein band at ~75 kDa was detected in the UVD seminal fluid (Lane 1) and band at ~80 kDa was detected in extracts of UVD wall (Lane 2). Abundant immuno-positive material with molecular weight between 75–80 kDa was detected in the extracts of whole testes (Lane 3). YP2 antibody did not give substantial immuno-positive signal in the lumen of seminal vesicles (SV, Lane 4), extracts of SV wall (Lane 5), hemolymph (Lane 6), flight muscles (Lane 7) or fat body (Lane 8). Equal amounts of total protein extracts (1 OD) were loaded in each lane.