Figure 1
From: Yolk protein is expressed in the insect testis and interacts with sperm
Glycoprotein detection in the UVD seminal fluid of Spodoptera littoralis. Proteins obtained from the UVD lumen were separated by 2-D gel electrophoresis and probed with Pro-Q Emerald 300 Glycoprotein Gel Stain. Black arrows indicate most abundant glycoprotein spots with isoelectric point (pI) between 6 and 7 (numbered 1–3). These proteins were submitted to mass spectrometry analysis, which detected in proteins #1 and #2 several peptides with homology to proteins that form yolk in the oocytes.