Figure 6

Wnt signalling components differentially required for generation of mesoderm from the late primitive streak. Timelapse imaging of embryos electroporated and grafted into unlabelled host embryos with GFP over 20 hours – intervals of 2.5 hours are shown. GFP control cells grafted into the anterior primitive streak(A) or into the posterior primitive streak (D). Cells expressing pCAβ-IRES-GFP-DshΔPDZ/DEP grafted into the anterior streak (B) or into the posterior streak (E). Cells expressing pCAβ-IRES-GFP-Pk1ΔPET/LIM grafted into the anterior streak (C) or into the posterior streak (F). Arrows show the position of the most recently formed somite. Arrowheads indicate the anterior-most GFP positive cells.