Figure 1

Pax6 is overexpressed in the eye of PAX77^+/+ embryos. Horizontal sections through the eye of wild-type and PAX77^+/+ embryos at (A) E12.5 and (B) E14.5, showing the expression of Pax6/PAX6. (C) Levels of endogenous Pax6 mRNA and total Pax6/PAX6 mRNA in the retina of E14.5 PAX77^+/+ embryos, relative to wild-types, determined by real time quantitative RT-PCR and normalised against GAPDH mRNA levels (n = 3 in each case).Pax6 mRNA levels are significantly decreased in the retinae of PAX77^+/+ embryos compared to those of wild-types (Student's t-test, p = 0.014) while Pax6/PAX6 mRNA levels are significantly increased (Student's t-test, p < 0.001). Scale bars: A, 200 μm; B, 500 μm.