Figure 4
From: Expression profiling in transgenic FVB/N embryonic stem cells overexpressing STAT3
Immunohistochemical analysis of WT E14 ES cells and E14 overexpressing, Nanog, Pem and Pramel7. IHC analysis with OCT-3/4 and SSEA-1 and staining for alkaline phosphatase were performed after cultivation of the cells for 8 days without adding LIF to the medium. E14 ES cells differentiated under these conditions and lost the expression of OCT-3/4 and SSEA-1 and alkaline phosphatase. Both Pem/Rhox5 and Pramel7 overexpressing cells maintained the expression of the pluripotency markers. Nanog overexpressing cells, used as a control for the experiment, as expected maintained their pluripotency. Scale bar in both large and small panels: 100 μm.