Figure 3

In situ hybridization of the eight selected genes. A. Dppa3, Eif2s2, Pem/Rhox5 and Pramel6 exhibited a general expression in all cells of the morula and of the blastocyst. Expression of Hexokinase II, Lefty2 and PP1s15B was restricted to the cells of the inner part of the morula and to the ICM in the blastocyst. Sense probes in the same concentration of the antisense probes were used as negative controls for the hybridization. B. In situ hybridization with a Pramel7 antisense riboprobe of preimplantation embryos (a: two-cell embryo, b: four-cell embryo, c: eight-cell embryo; d, e: compacted morula, f, g: blastocyst, h: negative control with sense riboprobe). Magnification 40×.