Figure 5

Sheath cells projections extend into the rachis in fli-1 and let-60. (A-F) DIC and lim-7::gfp expression in wild-type, fli-1(ky535), and let-60(n2021) (the same animals at the same focal planes are shown). (A), (B) No lim-7::gfp is observed in the rachis of wild-type (arrows). (C-F) Arrows point to displaced nuclei (in the DIC images) and associated fingers of lim-7::gfp expression in the rachis of these mutants. (G-I) Merged confocal micrographs of lim-7::gfp expression (green) and DAPI nuclear staining (blue) in dissected gonads. In lfi-1(ky535) and let-60(n2021), extensions of lim-7::gfp are observed associated with displaced nuclei in the rachis (arrows). The scale bar in (A) applies to (A-F); the scale bar in (G) applies to (G-I).