Figure 8

The CBM and J-domains are necessary for dAux function in Notch signaling. (A) A schematic diagram depicting various mRFP-tagged dAux constructs (see text for explanation). (B) A Western blot of extracts from flies expressing dAux^FL-mRFP (lane 1), dAux^ΔK-mRFP (lane 2), dAux^CJ-mRFP (lane 3), dAux^ΔC- mRFP (lane 4), and dAux^ΔJ-mRFP (lane 5) under the control of Act5C-Gal4. The blot was stained with anti-DsRed antibody, which recognizes the mRFP tag. The sizes of protein standards (in kD) are indicated on the left. (C-G) Projected spinning disk confocal images of larval eye discs from (C) Act5C>FLP/UAS-dAux^FL-mRFP; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nls, (D) Act5C>FLP/UAS-dAux^ΔK-mRFP; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nls, (E) Act5C>FLP/UAS-dAux^CJ-mRFP; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nls, (F) Act5C>FLP/UAS-dAux^ΔC-mRFP; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nls, and (G) Act5C>FLP/UAS-dAux^ΔJ-mRFP; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nls. These eye discs were stained with α-Elav antibody (red), and dAux^F956* mutant clones are indicated by the absence of nuclear GFP (green) and outlined by white dotted lines. The fluorescence from mRFP was not shown to present a clearer view of the organization of Elav-positive cells in dAux clones. Scale Bar, 10 μm.