Figure 4

Double and triple staining of ED 11.5 (A) and ED 13.5 (B – F) mouse embryos with Prox1 (red), Lyve-1 (blue), and F4/80 (green). A) Cell showing co-expression of Prox1 and F4/80 (arrow), whereas another cell is only F4/80 positive. B) Overview showing the jugular lymph sac (jls) and the cardinal vein (v). Prox1 and Lyve-1 are co-expressed in LECs of the jls. Arrow indicates integration of F4/80⁺ cell into the jls. C) Higher magnification of B) showing triple positive cells of the JLS. D) Prox1, E) Lyve-1, F) F4/80.