Figure 2

Double staining of scattered mesodermal cells with pan-endothelial, lymphendothelial and macrophage markers of ED 10 (A, B), ED 12.5 (D, E) and ED 13.5 (C, F, G) mouse embryos. A) Two mesenchymal cells of the dermatome (arrows) which are located at a great distance from the cardinal vein (v) express CD31 (green) and Prox1 (red). nt, neural tube. B) Higher magnification of A). Note weak CD31 expression of Prox1⁺ mesenchymal cells (arrows) as compared to blood vessels (bv). C) Cells double positive for lymphendothelial markers LA102 (green) and Prox1 (red), and D) LA102 (green) and Lyve-1 (red). E) Cells double positive for macrophage marker CD11b (green) and lymphendothelial marker Prox1 (red). F) Some cells co-express (yellow) CD11b (green) and Lyve-1 (red) in the mesenchyme adjacent to the JLS, and G) in the dermis, where a cell positive for both markers (arrow) seems to be integrated into a lymph vessel (lv).