Cloning and characterization of microRNAs from rainbow trout (Oncorhynchus mykiss): Their expression during early embryonic development

Table 1 Cloned rainbow trout miRNAs and primers used for real time PCR analysis.

MicroRNA	Sequence	Size	Real Time Primer Sequence	Conserved in other animals
omy-miR-21	UAGCUUAUCAGACUGGUGUUGGC	23	TAGCTTATCAGACTGGTGTTGGC	dre, fru, tni
omy-miR-21t	UAGCUUAUCAGACUGGUGUUGAC	23	TAGCTTATCAGACTGGTGTTGAC	see Figure 2A
omy-miR-23a	AUCACAUUGCCAGGGAUUUCCA	22	ATCACATTGCCAGGGATTTCCA	dre, fru, tni, bta
omy-miR-26a	UUCAAGUAAUCCAGGAUAGGCU	22	TTCAAGTAATCCAGGATAGGCT	dre, ssc, ptr, ggo, lla, mml, ppa, fru, tni, bta
omy-miR-30d	UGUAAACAUCCCCGACUGGAAG	22	TGTAAACATCCCCGACTGGAAG	bta, hsa, mmu, rno, gga dre, ptr, ggo, mne, ppa, fru, tni, xtr
omy-miR-92a	UAUUGCACUUGUCCCGGCCUGU	22	TATTGCACTTGTCCCGGCCTGT	dre, ggo, lme, age, ppa, ppy, ptr, mml, sla, lla, mne, fru, tni, bta
omy-miR-100t	AACCCGUAGAUCCGAAUUUGU	21	AACCCGTAGATCCGAATTTGT	see Figure 2B
omy-miR-125a	UCCCUGAGACCCUUAACCUGUG	22	TCCCTGAGACCCTTAACCTGTG	dre, fru, tni, xtr
omy-miR-125b	UCCCUGAGACCCUAACUUGUGA	22	TCCCTGAGACCCTAACTTGTGA	dre, dme, hsa, rno, gga, dps, aga, dre, ssc, ggo, ppa, ssc, age, ppy, ptr, mml, sla, lla,, mne, lca, fru, tni, bta, xtr, mdo
omy-miR-125t	UCCCUGAGACUUUAACUUGUGA	22	TCCCTGAGACTTTAACTTGTGA	see Figure 2C
omy-miR-126t	UCGUACCGUGAGUAAUAAUGCAC	23	TCGTACCGTGAGTAATAATGCAC	see Figure 2D
omy-miR-126*	CAUUAUUACUUUUGGUACGCG	21	CATTATTACTTTTGGTACGCG	mmu, hsa, rno, gga, dre, xtr
omy-miR-200b	UAAUACUGCCUGGUAAUGAUGAU	23	TAATACTGCCTGGTAATGATGAT	gga, xtr
omy-miR-455	UAUGUGCCCUUGGACUACAUCG	22	TATGTGCCCTTGGACTACATCG	dre, fru, tni, gga, xtr

ISSN: 1471-213X