Figure 3
Expression patterns of rainbow trout miRNAs (A to D), Stat3 (E) and Dicer (F) during early embryogenesis as revealed by real-time PCR analysis. Embryonic stages analyzed include unfertilized eggs (0-dpf), embryos at 1-dpf, 2-dpf, 3-dpf, 5-dpf and 6-dpf. Quantity of each miRNA or mRNA was normalized to Histone H2A. The means of the normalized gene expression values for each embryonic stage were calculated and expressed as relative fold changes (n = 3, mean ± SEM). Different letters indicate significant difference at P < 0.05.