Table 1 Response of primary osteoblasts and muscle fibroblasts to cyclic mechanical strain.

wt DT Os

-

82.3 ± 7.0 (91)

-

Bmp5^clvDT Os

-

52.2 ± 24.5 (80)

<0.001

wt FT Os

-

80.9 ± 8.5 (95)

-

Bmp5^clvFT Os

-

82.5 ± 6.5 (93)

NS

wt DM Fib

-

85.7 ± 3.8 (106)

-

Bmp5^clvDM Fib

-

84.6 ± 4.7 (101)

NS

wt DT Os

0

83.5 ± 5.7 (123)

-

0.1

80.2 ± 8.7 (148)

<0.01

1

71.1 ± 16.7 (111)

<0.001

10

62.3 ± 21.5 (107)

<0.001

Cell type

Time strained (min)

% Nuclear SMAD

P value ( wt vs. Bmp5 ^clv )

wt DT Os

0

7.1 ± 3.6 (94)

-

10

15.4 ± 6.4 (99)

-

20

34.2 ± 3.6 (139)

-

30

35.8 ± 8.5 (131)

-

60

32.4 ± 8.2 (124)

-

24 h

8.6 ± 3.1 (146)

-

Bmp5^clvDT Os

0

9.1 ± 4.6 (86)

NS

10

13.7 ± 5.2 (94)

NS

20

14.9 ± 5.5 (101)

<0.01

30

18.4 ± 4.3 (100)

<0.05

60

14.7 ± 1.6 (107)

<0.05

24 h

7.5 ± 4.5 (90)

NS

wt FT Os

0

7.9 ± 6.3 (101)

-

10

15.8 ± 8.6 (124)

-

20

31.2 ± 3.3 (123)

-

30

37.6 ± 1.3 (133)

-

60

30.0 ± 7.3 (149)

-

24 h

7.8 ± 4.3 (121)

-

Bmp5^clvFT Os

0

8.4 ± 2.7 (101)

NS

10

12.7 ± 7.9 (126)

NS

20

34.5 ± 6.1 (134)

NS

30

32.4 ± 5.0 (106)

NS

60

26.4 ± 9.6 (111)

NS

24 h

8.2 ± 3.0 (105)

NS