Figure 3

Nuclear teneurin-1 intracellular domain is observed in subsets of neurons and in specific tissues. Schematic diagram of teneurin domain architecture and antibodies used in this study (A). Comparison of immunostaining with antibodies to the extracellular and intracellular domains in sections and in vitro (B to I). Neurons of the piriform cortex at E17 (B, C) and neurons found in the retinal ganglion cell layer at E12 (D, E) stained with anti-ICD. The anti-ICD labels nuclei in the piriform cortex and labels a subset of retinal ganglion cells (arrow), but other TuJ1-positive neurons (label not shown) in the retinal ganglion cell layer are labeled with anti-ICD teneurin-1 but do not exhibit nuclear staining (arrowhead). There are also TuJ1-positive neurons in the retinal ganglion cell layer that are negative for teneurin-1 (asterisk). Head mesenchyme at E7 stained with anti-EGF (F, G) and anti-ICD (H, I). In the head mesenchyme, the anti-ICD labels the nucleus of most cells, whereas the anti-EGF exhibits cell-surface staining. Green, teneurin-1; blue (Hoechst), nuclei.